1. Introduction
- Welcome and brief overview of the post-mortem lineage tracing
- The Evolution of lineage tracing: Past, Present, and Future Perspectives
- Whole Genome human cell lineage tracing
2. Basic of clonal expansion
- Post-mortem biopsy, culture in general.
- Special consideration of post-mortem clonal expansion.
3. Basic of laser capture microdissection
- Post-mortem biopsy, histology in general.
- Special consideration of post-mortem laser capture microdissection.
4. Basic of whole genome sequencing lineage tracing
- lineage tracing in general.
- Special consideration of post-mortem lineage tracing.
5. Closing Remarks

Emerging technologies such as virtual reality (VR), augmented reality (AR) and mixed reality (MR) hold transformative potential in education and healthcare. The curriculum included lectures and hands-on sessions focusing on the application of virtual reality and artificial intelligence technology in anatomy education and clinical simulations. The attendee will have hands-on experience on virtual dissection table (MDBOX®, MEDICAL IP Co., Ltd., Seoul, Korea), VR headsets (Oculus Quest 2, Meta Quest, Menlo Park, CA, USA), an AR headset (HoloLens 2, Microsoft, Redmond, WA, USA). The attendee will learn how to use medical image processing software (MEDIP PRO®, MEDICAL IP Co., Ltd., Seoul, Republic of Korea). Through the experience of both hardware and software of 3D medical images, the attendee will be equipped with the capacity to organize educational course based on 3D hardware and software for anatomy education and clinical simulation.

1. Introduction
- Welcome and brief overview of the workshop
2. Tissue Preparation
- Overview of tissue preparation techniques for neuroscience research
- Practical tips and demonstrations
3. In Situ Hybridization Techniques
- Principles and applications of in situ hybridization
4. Immunofluorescence Techniques
- Immunofluorescence applications in neuroscience
5. Spatial Transcriptomics in Neuroscience
- Introduction to spatial transcriptomics
6. Closing Remarks

1. Introduction
- Welcome and brief overview of the workshop
- The Evolution of Anatomical Research: Past, Present, and Future Perspectives
2. Immersion in Topographical Anatomy
- Ultrasonography-based Analysis of Anatomical Structures
- Face Painting as an Ultrasonography-based Anatomical Learning Tool
3. Clinical Implication
4. Innovative Approaches to Three-Dimensional Anatomical Data Generation
- Challenges in Constructing Three-Dimensional Anatomical Data through Ultrasound and Motion Capture Technology
5. Closing Remarks

Purpose
In this program, we aim to demonstrate not only the traditional Sihler’s whole mount nerve staining technique but also a ‘modified technique’ for vascular research. Through this program, participants will have the opportunity to gain hands-on experience, learn experimental techniques, and engage in knowledge sharing and problem-solving among participating anatomists. Furthermore, we intend to facilitate discussions about potential advancements in the Sihler’s staining technique for the future.

Significance
Sihler’s whole mount nerve staining is an experimental technique that allows for the examination of intramuscular nerve distribution. This long-standing traditional method has regained attention recently due to the active use of botulinum neurotoxin injections for cosmetic and rehabilitation purposes. This program enables participants to gain practical experimental experience by conducting Sihler’s whole mount nerve staining.

Programs
1. Preparation of reagents required for Sihler's staining
2. Step-by-Step demonstration and observation of Sihler's staining
3. Demonstration of latex injection into blood vessels
4. Transparency after latex injection: Step-by-Step specimen preparation
5. Observation of specimens after latex injection and Sihler’s staining